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Cercospora beticola, is one of the most widespread foliar diseases of sugar beet. A decrease in the susceptibility of C. beticola to azoles, a class of fungicides that inhibit the biosynthesis of sterol by acting on sterol 14-demethylase, has been reported in France over the past decade. The contribution of the overexpression of the Cyp51 gene, coding for the sterol 14-demethylase, has been studied on a set of 27 isolates characterized for their sensitivity to azoles and sequenced for the Cyp51 gene.