JobPostdoctoral position in ion channels and pain (M/F)

About

Missions

The postdoctoral researcher's responsibilities (4-month contract) will include the design, execution, and analysis of experiments within the framework of research projects related to acute and chronic pain. The main objective of the project is to study the potential role of certain ion channels in the generation and transmission of pain signals. The laboratory has various knockout and knock-in mouse models for certain genes encoding potassium channels of the two-pore channel family. One of the postdoctoral researcher's tasks will be to perform sensitivity tests to mechanical or thermal stimulation, for example, on these mouse lines. Other tests will also be carried out to evaluate other sensory and cognitive functions. Another task will involve establishing primary cultures of neurons from these different animal lines to perform electrophysiological recordings. Different pain models will be created after injections of inflammatory substances, and spontaneous and evoked pain tests will be performed. Finally, another mission will consist of performing histological sections of mouse nerve tissue to carry out immunological or RNAscope staining.
This entire work is part of a pre-clinical approach to identifying new therapeutic targets for the treatment of pain.

Activities

– Electrophysiological recordings (patch clamp and TEVC)
– Behavioral tests on mice to quantify cognitive functions, motor skills, and pain.
– Perform interventions on mice such as: surgery, injections, sampling, labeling, etc.
– Formatting of results and statistical analysis.
– Monitoring of literature and improvement/adaptation of techniques and analyses according to research projects.
– Writing scientific articles.

SKILLS

– Cell culture and microscopy
– Molecular biology and cloning
– Electrophysiology on Xenopus cells or oocytes
– Surgery in mice.
– To know and be able to implement behavioral tests in mice.
– Ability to analyze data: statistical analysis, electrophysiological signal analysis.
– Histology, immunohistochemistry.
– Training in animal experimentation and surgery.
– Sense of organization, rigor and method, motivation, autonomy, team spirit.
– Knowledge of written and spoken English.

Work context

The position is available at the Institute of Molecular and Cellular Pharmacology (IPMC) in Valbonne – Sophia Antipolis (Alpes-Maritimes). The IPMC is a joint research unit (UMR 7275) established between the CNRS, Inserm, and Université Côte d'Azur (250 people, 8,000 m² of buildings). Its 20 research teams benefit from a high-level technological environment in integrative biology, electrophysiology, molecular and cellular biology, imaging, cytometry, biomolecule analysis, and functional genomics. In particular, the shared technical platforms for animal experimentation and the team's own equipment allow for detailed phenotyping of mice. The postdoc will work in the "Physiology and Pathophysiology of Potassium Channels" team under the supervision of Delphine Bichet and Franck Chatelain.
The position is located in a sector related to the protection of scientific and technical potential (PPST), and therefore requires, in accordance with regulations, that your arrival be authorized by the competent authority of the MESR.

The position is located in a sector related to the protection of scientific and technical potential (PPST), and therefore requires, in accordance with regulations, that your arrival be authorized by the competent authority of the MESR.

Apply via the CNRS Job Portal:

https://emploi.cnrs.fr/Offres/CDD/UMR7275-DELCHA-003/Default.aspx