Mammalian 2P channels Putative assembly



Molecular properties of mammalian cloned 2P domain K+ channels


Last update: 26 / 4/ 2004


Table of contents


Structural subfamilies of mammalian 2P domain K+ channels

TWIK subfamily TREK subfamily TASK subfamily K2p5.1, TASK-2 subfamily THIK subfamily TRESK subfamily

KCNK1 (K2p1.1, TWIK-1)

KCNK2 (K2p2.1, TREK-1)

KCNK3 (K2p3.1, TASK-1, TBAK-1, OAT-1)

KCNK5 (K2p5.1, TASK-2)

KCNK13 (K2p13.1, THIK-1)

KCNK18 (TRESK)

KCNK6 (K2p6.1, TWIK-2, TOSS)

KCNK4 (K2p4.1, TRAAK, KT4.1)

KCNK9 (K2p9.1, TASK-3, KT3.2)

KCNK16 (K2p16.1, TALK-1)

KCNK12 (K2p12.1, THIK-2)

 

KCNK7 (K2p7.1)

KCNK10 (K2p10.1, TREK-2)

KCNK15 (K2p15.1, TASK-5, KT3.3)

KCNK17 (K2p17.1, TALK-2, TASK-4)

.  

See the HUGO Gene Nomenclature Committee

 

Channel names based on: International Union of Pharmacology (IUPHAR): "Compendium of Voltage-gated Ion Channels", IUPHAR Media Editor

 

See my page on the Nomenclature of the K+ channels superfamily

 

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Dendrogram (human sequences)


Produced by Treeview using a ClustalX alignment.

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Genomic properties of mammalian 2P domain K+ channels

Human genes Channel names (IUPHAR) Other names Human chromosomal localisation NCBI accession number (human) Other cloned species Original cloning article References
KCNK1 K2p1.1 TWIK-1 1q42-q43 U33632 Mouse, Rat Lesage et al. (1996) List of references
KCNK2 K2p2.1 TREK-1 1q41 AF129399 Mouse, Rat Fink et al. (1996) List of references
KCNK3 K2p3.1 TASK-1
(TBAK-1, OAT1)
2p23 AF006823 Rat, Mouse Duprat et al. (1997) List of references
KCNK4 K2p4.1

TRAAK
(KT4.1)

11q13 NM_016611 Mouse Fink et al. (1998) List of references
KCNK5 K2p5.1 TASK-2 6p21 AF084830 Mouse Reyes et al. (1998) List of references
KCNK6 K2p6.1 TWIK-2
(TOSS)
19q13.1 AF117708 Mouse, Rat Chavez et al. (1999) List of references
KCNK7 K2p7.1 KCNK7 11q13 AF110522 Mouse Salinas et al. (1999) List of references
KCNK9 K2p9.1 TASK-3
(KT3.2)
8q24.3 NM_016601 Guinea pig, Rat Kim et al. (2000) List of references
KCNK10 K2p10.1 TREK-2 14q31 AF279890 Rat Bang et al. (2000) List of references
KCNK12 K2p12.1 THIK-2 2p22-2p21 AF287302 Rat Rajan et al. (2000) List of references
KCNK13 K2p13.1 THIK-1 14q24.1-14q24.3 AF287303 Rat Rajan et al. (2000) List of references
KCNK15 K2p15.1 TASK-5
(KT3.3)
20q12 AF336342_1 -

Kim & Gnatenco (2001)

List of references
KCNK16 K2p16.1 TALK-1 6p21 AL136087 - Girard et al. (2001) List of references
KCNK17 K2p17.1 TASK-4
(TALK-2)
6p21 AL136087 - Decher et al. (2001) List of references
KCNK18 - TRESK 10 - -

Sano et al. (2003)

List of references

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Map of human chromosomal localisation

Chromosomal localisation

From the NCBI Genome database


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Structural properties of mammalian 2P domain K+ channels

Human genes Channel names (IUPHAR) Other names Amino acids P1 motif P2 motif PKA sites PKC sites Casein kinase sites Cam kinase sites Tyrosine kinase sites Glycosylation sites References
KCNK1 K2p1.1 TWIK-1 336 GYG GLG . 1 1 1 . 1 List of references
KCNK2 K2p2.1

TREK-1 (short)

411

GFG GFG 2 4 2 . . 2 List of references
KCNK2 K2p2.1 TREK-1 (long) 426 GFG GFG 2 4 2 . . 2 List of references
KCNK3 K2p3.1 TASK-1
(TBAK-1, OAT1)
394 GYG GFG 2 2 3 . 1 1 List of references
KCNK4 K2p4.1 TRAAK (short), KT4.1a 393 GYG GFG 1 6 3 . . 2 List of references
KCNK4 K2p4.1 TRAAK (long), KT4.1b 419 GYG GFG 1 6 3 . . 2 List of references
KCNK5 K2p5.1 TASK-2 499 GYG GFG . . . . . 1 List of references
KCNK6 K2p6.1 TWIK-2
(TOSS)
313 GYG GLG . 1 1 . . 1 List of references
KCNK7 K2p7.1 KCNK7 307 GYG GLE . 1 5 . . 1 List of references
KCNK9 K2p9.1 TASK-3,
KT3.2
374 GYG GFG 1 / 3 2 / 3 2 . . 1 List of references
KCNK10 K2p10.1 TREK-2 (short), TREK-2a 538 GYG GFG 1 1 . . . 2 List of references
KCNK10 K2p10.1 TREK-2 (long), TREK-2b 543 GYG GFG 1 2 . . . 2 List of references
KCNK10 K2p10.1 TREK-2 (long), TREK-2c 543 GYG GFG 1 2 . . . 2 List of references
KCNK12 K2p12.1 THIK-2 430 GFG GFG 2 8 3 . . 1 List of references
KCNK13 K2p13.1 THIK-1 408 GFG GFG 2 4 5 . . 2 List of references
KCNK15 K2p15.1 TASK-5
(KT3.3)
330 GYG / EYG GFG 2 1 . . . . List of references
KCNK16 K2p16.1 TALK-1 309 GYG GFG 1 2 . . . 2 List of references
KCNK17 K2p17.1 TALK-2
(TASK-4)
332 GYG GFG . 2 . . . 2 List of references
KCNK18 - TRESK 385 GYG GFG . . . . . 2 List of references
Human genes Channel names (IUPHAR) Names Amino acids P1 motif P2 motif PKA sites PKC sites Casein kinase sites Cam kinase sites Tyrosine kinase sites Glycosylation sites References

Legend: /: various results according to the study

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Selectivity filter

The amino acid motif that composes the classical selectivity filter of a potassium channel is GYG.

The 2P domain K+ channel present two motifs: P1 and P2.
Most P1 contain a GYG motif.

G Glycine Y Tyrosine G Glycine
G Glycine Y Tyrosine G Glycine

The first exceptions are KCNK2 (K2p2.1, TREK-1), KCNK13 (K2p13.1, THIK-1), and KCNK12 (K2p12.1, THIK-2) that contain a GFG motif in their P1:

G Glycine F Phenylalanine G Glycine
G Glycine F Phenylalanine G Glycine

Another exception is KCNK15 (K2p15.1, TASK-5) that contain a EYG motif :

E Aspartate Y Tyrosine G Glycine
E Aspartate Y Tyrosine G Glycine

Most P2 contain a GFG, except KCNK1 (K2p1.1, TWIK-1) and KCNK6 (K2p6.1, TWIK-2) that contain an unusual GLG:

G Glycine L Leucine G Glycine
G Glycine L Leucine G Glycine

The most unusual motif is that of KCNK7 (K2p7.1) with a GLE in its P2 motif. Please note that this channel does not functionally express.

G Glycine L Leucine E Glutamate
G Glycine L Leucine E Glutamate

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Proteic sequences similitude (%)


Names KCNK1
(K2p1.1, TWIK-1)
KCNK2
(K2p2.1, TREK-1)
KCNK3
(K2p3.1, TASK-1, TBAK-1, OAT-1)
KCNK4
(K2p4.1, TRAAK, KT4.1)
KCNK5
(K2p5.1, TASK-2)
KCNK6
(K2p6.1, TWIK-2, TOSS)
KCNK7 KCNK9
(K2p9.1, TASK-3, KT3.2)
KCNK10
(K2p10.1, TREK-2)
KCNK12
(K2p12.1, THIK-2)
KCNK13
(K2p13.1, THIK-1)
KCNK15
(K2p15.1, TASK-5, KT3.3)
KCNK16
(K2p16.1, TALK-1)
KCNK17
(K2p17.1, TALK-2, TASK-4)

KCNK?
(
TRESK)

Names
KCNK1
(K2p1.1, TWIK-1)
100% 22.6 21.4 22.3 21.1 39.3 35.5 19.6 25.6 22.6 21.7 20.9 26.9 20.5 18.8
KCNK1
(K2p1.1, TWIK-1)
KCNK2
(K2p2.1, TREK-1)
22.6 100% 21.6 38.2 20.7 23 21.5 21.9 56.7 19 18.1 20.9 29.4 25.6 16.9
KCNK2
(K2p2.1, TREK-1)
KCNK3
(K2p3.1, TASK-1, TBAK-1, OAT-1)
21.4 21.6 100% 21.4 18.5 21.4 21.5 59.4 20.1 20.1 22.1 51.8 25.9 19.3 16.1
KCNK3
(K2p3.1, TASK-1, TBAK-1, OAT-1)
KCNK4
(K2p4.1, TRAAK, KT4.1)
22.3 38.2 21.4 100% 23.2 29.4 22.8 21.4 39.9 21.1 19.8 24.2 30.4 29.5 14.3
KCNK4
(K2p4.1, TRAAK, KT4.1)
KCNK5
(K2p5.1, TASK-2)
21.1 20.7 18.5 23.2 100% 23.6 18.9 20.9 20 17.7 13.2 23.3 30.7 29.5 13.5
KCNK5
(K2p5.1, TASK-2)
KCNK6
(K2p6.1, TWIK-2, TOSS)
39.3 23 21.4 29.4 23.6 100% 28.7 18.8 23.3 22.7 20.8 21.1 26.5 24.9 17.6
KCNK6
(K2p6.1, TWIK-2, TOSS)
KCNK7
(K2p7.1)
35.5 21.5 21.5 22.8 18.9 28.7 100% 19.5 22.1 20.5 16 22.8 22.5 20.5 16.3
KCNK7
(K2p7.1)
KCNK9
(K2p9.1, TASK-3, KT3.2)
19.6 21.9 59.4 21.4 20.9 18.8 19.5 100% 19.5 19 20.9 53 24.3 19 16
KCNK9
(K2p9.1, TASK-3, KT3.2)
KCNK10
(K2p10.1, TREK-2)
25.6 56.7 20.1 39.9 20 23.3 22.1 19.5 100% 19.1 19.9 21.5 30.7 25 18.2
KCNK10
(K2p10.1, TREK-2)
KCNK12
(K2p12.1, THIK-2)
22.6 19 20.1 21.1 17.7 22.7 20.5 19 19.1 100% 62.7 22.7 23.9 19.9 14.3
KCNK12
(K2p12.1, THIK-2)
KCNK13
(K2p13.1, THIK-1)
21.7 18.1 22.1 19.8 13.2 20.8 16 20.9 19.9 62.7 100% 21.8 23.9 19 14.1
KCNK13
(K2p13.1, THIK-1)
KCNK15
(K2p15.1, TASK-5, KT3.3)
20.9 20.9 51.8 24.2 23.3 21.1 22.8 53 21.5 22.7 21.8 100% 25.2 21.2 15.5
KCNK15
(K2p15.1, TASK-5, KT3.3)
KCNK16
(K2p16.1, TALK-1)
26.9 29.4 25.9 30.4 30.7 26.5 22.5 24.3 30.7 23.9 23.9 25.2 100% 36.2 19.7
KCNK16
(K2p16.1, TALK-1)
KCNK17
(K2p17.1, TALK-2, TASK-4)
20.5 25.6 19.3 29.5 29.5 24.9 20.5 19 25 19.9 19 21.2 36.2 100% 15.1
KCNK17
(K2p17.1, TALK-2, TASK-4)
KCNK18
(
TRESK)
18.8 16.9 16.1 14.3 13.5 17.6 16.3 16 18.2 14.3 14.1 15.5 19.7 15.1 100%
KCNK18
(
TRESK)
Names KCNK1
(K2p1.1, TWIK-1)
KCNK2
(K2p2.1, TREK-1)
KCNK3
(K2p3.1, TASK-1, TBAK-1, OAT-1)
KCNK4
(K2p4.1, TRAAK, KT4.1)
KCNK5
(K2p5.1, TASK-2)
KCNK6
(K2p6.1, TWIK-2, TOSS)
KCNK7 KCNK9
(K2p9.1, TASK-3, KT3.2)
KCNK10
(K2p10.1, TREK-2)
KCNK12
(K2p12.1, THIK-2)
KCNK13
(K2p13.1, THIK-1)
KCNK15
(K2p15.1, TASK-5, KT3.3)
KCNK16
(K2p16.1, TALK-1)
KCNK17
(K2p17.1, TALK-2, TASK-4)
KCNK?
(
TRESK)
Names

Sequences were compared using a Clustal method.

Legend: More than 50%, More than 30%, Less than 30%.

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Tissular distribution of mammalian 2P domain K+ channels


Legend:

Note: Some nice general studies have been performed on various channels, the results in the following tables arise from all other known papers and are plotted even in case of discrepancies between the sources.

Northern blot analysis: low level = Northern blot, high level = Northern blotNorthern blot

PCR analysis: low level = PCR, high level = PCRPCR

Tested tissues: h: human, m: mouse, r: rat, gp: guinea pig, .: not tested

Names Brain Heart Brain stem Spinal cord Cerebellum Aorta Lung Pulmonary artery Bone marrow Peripheral blood leukocytes Kidney Liver Small intestine Colon Squelettal muscle Names References
KCNK1
(K2p1.1, TWIK-1)
Northern blotNorthern blothm Northern blotNorthern bloth . . . . Northern blothm . . . Northern blothm Northern bloth . . Northern blotm KCNK1
(K2p1.1, TWIK-1)
List of references
KCNK2
(K2p2.1, TREK-1)
Northern blotNorthern blotm Northern blotm . . . . Northern blotNorthern blotm . . . Northern blotm . . . Northern blotm KCNK2
(K2p2.1, TREK-1)
List of references
KCNK3
(K2p3.1, TASK-1,
TBAK-1,
OAT1)
Northern blothm Northern blotNorthern blotm
Northern bloth
PCRPCRr
. . . . Northern bloth
Northern blotNorthern blotm
. . . Northern blothm . Northern blothm Northern bloth Northern blotm KCNK3
(K2p3.1, TASK-1,
TBAK-1,
OAT1)
List of references
KCNK4
(K2p4.1, TRAAK, KT4.1)
Northern blotNorthern blotm
PCRPCRhm
. PCRPCRm PCRPCRm PCRPCRm . . . . . PCRh . PCRh . . KCNK4
(K2p4.1, TRAAK, KT4.1)
List of references
KCNK5
(K2p5.1, TASK-2)
PCRm PCRm . . . . PCRPCRm . . . Northern blotNorthern bloth
PCRPCRm
Northern bloth
PCRPCRm
Northern bloth
PCRPCRm
PCRm PCRm KCNK5
(K2p5.1, TASK-2)
List of references
KCNK6
(K2p6.1, TWIK-2,
TOSS)
Northern bloth Northern blotNorthern bloth . . . PCRPCRhr Northern bloth
PCRPCRr
PCRPCRr PCRh Northern bloth
PCRh
Northern bloth
PCRPCRr
Northern bloth
Northern blotNorthern blotm
. Northern blotNorthern bloth . KCNK6
(K2p6.1, TWIK-2,
(TOSS)
List of references
KCNK7
(K2p7.1)
. PCRm PCRm . . . PCRPCRm . . . PCRm . PCRm PCRm . KCNK7
(K2p7.1)
List of references
KCNK9
(K2p9.1, TASK-3, KT3.2)
PCRPCRh,gp . . . PCRPCRhr . PCRr . . . PCRPCRr
PCRgp
PCRr . PCRr PCRr KCNK9
(K2p9.1, TASK-3, KT3.2)
List of references
KCNK10
(K2p10.1, TREK-2)
Northern blotNorthern blotr
PCRh
PCRh . . PCRPCRr . . . . . PCRPCRh PCRh PCRh PCRh . KCNK10
(K2p10.1, TREK-2)
List of references
KCNK12
(K2p12.1, THIK-2)
Northern bloth
PCRPCRh
Northern blotNorthern bloth
PCRh
. . . . Northern bloth
PCRPCRh
. . PCRh Northern bloth
PCRh
Northern bloth
PCRh
PCRh PCRh Northern blotNorthern bloth
PCRh
KCNK12
(K2p12.1, THIK-2)
List of references
KCNK13
(K2p13.1, THIK-1)
PCRPCRh PCRPCRh . . . . PCRPCRh . . . PCRPCRh PCRPCRh . . PCRh KCNK13
(K2p13.1, THIK-1)
List of references
KCNK15
(K2p15.1, TASK-5,
KT3.3)
Northern bloth PCRh . . . . PCRh . . . PCRh PCRh . . Northern blothPCRPCRh KCNK15
(K2p15.1, TASK-5,
KT3.3)
List of references
KCNK16
(K2p16.1, TALK-1)
. . . . . . . . . . . . . . . KCNK16
(K2p16.1, TALK-1)
List of references
KCNK17
(K2p17.1, TALK-2,
TASK-4)
. Northern bloth . . . . Northern bloth . . . . Northern bloth . . . KCNK17
(K2p17.1, TALK-2,
TASK-4)
List of references
KCNK18
(TRESK)
. . . PCRPCRh . . . . . . . . . . . KCNK18
(TRESK)
List of references
Names Brain Heart Brain stem Spinal cord Cerebellum Aorta Lung Pulmonary artery Bone marrow Peripheral blood leukocytes Kidney Liver Small intestine Colon Squelettal muscle Names References

Legend:

Northern blot analysis: low level = Northern blot, high level = Northern blotNorthern blot

PCR analysis: low level = PCR, high level = PCRPCR

Tested tissues: h: human, m: mouse, r: rat, gp: guinea pig, .: not tested

Names Pancreas Adrenal gland Thyroid gland Salivary gland Prostate Oesophagus Uterus Placenta Spleen Bladder Thymus Eye Ovary Skin Testis Stomach Names References
KCNK1
(K2p1.1, TWIK-1)
. . . . . . . Northern bloth . . . . . . . . KCNK1
(K2p1.1, TWIK-1)
List of references
KCNK2
(K2p2.1, TREK-1)
. . . . . . . . . . . . . . . . KCNK2
(K2p2.1, TREK-1)
List of references
KCNK3
(K2p3.1, TASK-1,
TBAK-1,
OAT1)
Northern blotNorthern bloth . . . Northern bloth . Northern bloth Northern blotNorthern bloth . . . . . Northern blotm Northern blotm Northern blotm KCNK3
(K2p3.1, TASK-1,
TBAK-1,
OAT1)
List of references
KCNK4
(K2p4.1, TRAAK, KT4.1)
. . . . PCRh . . PCRPCRh . . . PCRm . . PCRh . KCNK4
(K2p4.1, TRAAK, KT4.1)
List of references
KCNK5
(K2p5.1, TASK-2)
Northern blotNorthern bloth . . . . . . Northern bloth . . . . . . . . KCNK5
(K2p5.1, TASK-2)
List of references
KCNK6
(K2p6.1, TWIK-2,
TOSS)
Northern bloth . . PCRPCRh . PCRPCRh . Northern blotNorthern bloth
PCRPCRh
Northern blotNorthern bloth
PCRPCRhr
. Northern bloth
PCRh
. . . . PCRPCRhr KCNK6
(K2p6.1, TWIK-2,
TOSS)
List of references
KCNK7
(K2p7.1)
. . . PCRm . . PCRm . . PCRm PCRm PCRPCRm PCRm . PCRm PCRPCRm

KCNK7
(K2p7.1)

List of references
KCNK9
(K2p9.1, TASK-3, KT3.2)
. . . . . . . . PCRr . . . . . PCRr PCRr KCNK9
(K2p9.1, TASK-3, KT3.2)
List of references
KCNK10
(K2p10.1, TREK-2)
Northern blotNorthern blotr
PCRPCRh
. . . PCRh . . . PCRr . PCRh . . . PCRhr . KCNK10
(K2p10.1, TREK-2)
List of references
KCNK12
(K2p12.1, THIK-2)
Northern bloth
PCRh
. . . PCRh . . Northern bloth
PCRh
PCRPCRh . PCRh . PCRh . PCRh PCRh KCNK12
(K2p12.1, THIK-2)
List of references
KCNK13
(K2p13.1, THIK-1)
. . . . . . . . PCRPCRh . . . . . PCRPCRh PCRPCRh KCNK13
(K2p13.1, THIK-1)
List of references
KCNK15
(K2p15.1, TASK-5,
KT3.3)
Northern blotNorthern bloth
PCRh
Northern blotNorthern bloth
PCRPCRh
PCRh PCRh . . . Northern bloth . . . . PCRh . PCRPCRh . KCNK15
(K2p15.1, TASK-5,
KT3.3)
List of references
KCNK16
(K2p16.1, TALK-1)
Northern blotNorthern bloth . . . . . . . . . . . . . . . KCNK16
(K2p16.1, TALK-1)
List of references
KCNK17
(K2p17.1, TALK-2,
TASK-4)
Northern blotNorthern bloth . . . . . . Northern bloth . . . . . . . . KCNK17
(K2p17.1, TALK-2,
TASK-4)
List of references
KCNK18
(TRESK)
. . . . . . . . . . . . . . . . KCNK18
(TRESK)
List of references
Names Pancreas Adrenal gland Thyroid gland Salivary gland Prostate Oesophagus Uterus Placenta Spleen Bladder Thymus Eye Ovary Skin Testis Stomach Names References

Legend:

Northern blot analysis: low level = Northern blot, high level = Northern blotNorthern blot

PCR analysis: low level = PCR, high level = PCRPCR

Tested tissues: h: human, m: mouse, r: rat, gp: guinea pig, .: not tested

 

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Auxiliary subunits of mammalian 2P domain K+ channels

 

Names of K2P channels Channel interaction sites Auxiliary subunits References
KCNK3, K2p3.1, TASK-1 (human) SSV (positions 392-394) p11 (annexin II subunit)

Abstract NCBIGirard et al. (2002)

KCNK3, K2p3.1, TASK-1 (rat)
KCNK9, K2p9.1, TASK-3 (rat)
KRRSSV (positions 407-411)
IRRKSI (positions 407-411)
14-3-3
14-3-3
Abstract NCBIRajan S et al. (2002)
KCNK3, K2p3.1, TASK-1 (rat)

KR (positions 2-3)
RRSSV (positions 390-394)

beta-COP
14-3-3beta
Abstract NCBIO'Kelly et al. (2002)
KCNK3, K2p3.1, TASK-1 (rat) N-terminal 70 residues HIV-1 Vpu

Abstract NCBIHsu K et al. (2004)

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Mammalian 2P channels Putative assembly